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报告题目：Effects of Electric Fields on the Release and Content of Extracellular Vesicles
报告时间：2024-11-13 10:00
报告人： 王海龙 研究员
美国梅奥诊所
报告地点：卢嘉锡楼202报告厅
转播地点：翔安校区能原材料大楼三号楼会议室2，漳州校区生化主楼307教室
报告摘要：
Extracellular vesicles (EVs) are small membrane-bound structures that originate from various cell types and carry molecular cargos to influence the behavior of recipient cells. The use of EVs as biomarkers for diagnosis and as delivery vehicles for treatment in a wide range of human disease is a rapidly growing field in research and clinical practice. We hypothesized that electric fields (EFs) could influence the release and content of EVs. To examine this hypothesis, we developed a specialized bioreactor enabling cells to thrive in a three-dimensional setting, replicating in-vivo conditions amidst programmable EF environments. We established a three-step EV purification protocol to achieve high-density production of EVs. We also performed mass spectrometry-based proteomics analysis on EV-carrying proteins and used high-resolution nanoparticle flowcytometry for single vesicle analysis. Findings from this report suggest that electrical stimulation, employing physiologically relevant amplitudes typical in therapeutic deep brain stimulation, influences the release of EVs and their cargo content in a frequency-dependent fashion. This conclusion could carry significant implications for both fundamental biological understanding and medical advancements. First, it raises an intriguing question about how the endogenous electrical activity of neuronal and other cellular assemblies influence the production and composition of EVs. Second, it reveals a novel underlying mechanism of how therapeutic electrical stimulations can modulate EVs and treat human brain disorders. Third, it provides a novel approach to utilize electrical stimulation for generating desired EV cargos in a programmable setting.
报告人简介：
王海龙博士于1987年和1994年分别获得复旦大学生物物理学学士学位和中国科学院上海生理研究所神经生物学博士学位。自1995年开始，他在美国明尼苏达州罗切斯特市的梅奥诊所生理科和神经科从事科学研究，历任博士后和研究科学家，获得多项NIH R01资助，并在Nature Neuroscience, Neuron, J. Bio. Chem等期刊发表了40余篇论文，同时撰写了多本书籍章节。王博士的研究团队专注于研究电磁场对生物功能的调节，目前的研究重点在于通过新颖技术提升对电磁场与细胞外囊泡相互作用的理解。研究结果显示，具有治疗用途的深部脑刺激所使用的生理相关幅度的电刺激，会以频率依赖的方式影响EVs的释放及其内容物的特性。这揭示了电刺激如何调节EVs并治疗脑疾病的潜在机制，同时提供了一种在可编程环境中利用电刺激生成特定EV货物的新方法，这对于基础生物学理解和医学进步具有重要意义。
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